粮食及其制品中合成着色剂检测方法的改进

Improvement of Testing Method of Synthetic Colors in Cereals and Their Products

摘要:

以甲醇氨水水作为提取液，对绿豆、小米、紫米等有色粮食及方便面、挂面等粮食制品中的色素进行提取，提取液经过浓缩、定容和过滤后进行高效液相色谱测定，以甲醇和0.02mol·L-1乙酸铵溶液进行梯度洗脱，采用多波长（428, 482, 254, 510, 600nm）同时测定提取液中的柠檬黄、日落黄、胭脂红、苋菜红、亮蓝。结果表明，该法对柠檬黄、日落黄、胭脂红、苋菜红和亮蓝的检出限分别为1.01, 1.70, 4.38, 1.14, 5.32ng，平均回收率达到82%～102%，相对标准偏差为0.6%～5.3%。该方法操作简单，节省时间，同时采用多波长测定方法减少了其他组分的干扰，提高了测定的灵敏度。

Abstract:

Pigments in cereal products including mung bean, millet, purple rice, fine dried noodles and instant noodles were extracted using methanol-ammonia-water as the solvent. High performance liquid chromatography (HPLC) was used to determine the concentration of tartrazine, sunset yellow, carmine, amaranth, and brilliant blue in the extracts simultaneously by the multi-wavelength (428, 482, 254, 510nm, and 600nm) method, using methanol and 0.02mol·L-1 ammonium acetate as the gradient elution. The results showed that the detection limits of tartrazine, sunset yellow, carmine, amaranth, and brilliant blue were 1.01, 1.70, 4.38, 1.14ng, and 5.32ng, respectively, the average recovery was around 82%~102%, and the relative standard deviation (RSD) was 0.6%~5.3%. This method is simple, time-saving, and the sensitivity is improved for the multi-wavelength treatment reduces the disturbance of other components.