运动发酵单胞杆菌蛋白质双向电泳技术的建立

Establishment of Two-dimensional Gel Electrophoresis Technology on Zymomonas Mobilis

摘要:

运动发酵单胞杆菌（Zymomonas mobilis）具有高乙醇耐受力和高乙醇转化率，产醇率也较高，是一种很好的生物能源。运用双向电泳技术对运动发酵单胞杆菌蛋白质组进行了一系列单因子实验，经过验证获得了重复性好、分辨率高的双向电泳图谱，为该菌的比较蛋白质组学的研究，发现并克隆产乙醇率高的基因奠定了基础。

Abstract:

Zymomonas mobilis Can be useful as bioenergy with high ethanol tolerance, high ethanol conversion, and high ethanol production. The influencing factors were studied about Two-dimensional gel electrophoresis technology on Zymomonas mobile, and the optimized conditions for 2-DE map were established. The result showed good repeatability and high resolution. This technology built the foundation of comparative proteomic analysis and clonging of the high ethanol producing ratio gene.