1株桃果实采后病原真菌的鉴定 以及碳源代谢指纹图谱分析

Identification and Carbon Metabolic Fingerprinting Analysis of a Pathogen Isolated from Postharvest Peach Fruit

摘要:

从采后贮藏过程中发病的八月脆桃果实(Amygdalus persicacv.Bayuecui)中分离到1株真菌菌株074#。通过对病原菌形态学观察以及核糖体ITS rDNA序列分析,证明菌株074#为灰葡萄孢霉菌（Botrytis elliptica) 。致病性试验结果表明,桃果实为灰葡萄孢霉菌的寄主。通过Biolog FF MicroPlate分析该病原菌对95种碳源的利用能力,结果表明,Botrytis elliptica代谢指纹图谱为最适碳源包括D-果糖、蔗糖、L-苹果酸、琥珀酸、D-葡萄糖酸、L-天门冬氨酸、L-丝氨酸、腺苷、5′-磷酸腺苷等26种,可利用碳源14种,不可利用碳源55种。

Abstract:

A fungal pathogen, strain 074#, was isolated from infected peach fruit during postharvest storage periods. Morphological characterization and ITS rDNA analysis confirmed that the strain 074# was Botrytis elliptica. The pathogenicity tests confirmed that Amygdalus persica is a natural host of Botrytis elliptica. The Biolog Microbial Identification System with FF MicroPlate was applied for carbon source utilization test of Botrytis elliptica based on their abilities to utilize 95 discrete substrates. And the result showed that the carbon metabolic fingerprinting of isolated strain was composed of 26 optimal carbon sources, including D-fructose, sucrose, L-malic acid, succinic acid, D-gluconic acid, L-aspartic acid, L-serine, adenosine, denosine-5'-monophosphate, as well as 14 available carbon sources and 55 unavailable carbon sources.