Total of 51and 54 volatile compounds of mechanically extracted juice and hand extracted juice were analyzed by solid phase microextraction combined with gas chromatography-mass spectrometry. The total peak area of mechanically extracted juice was higher than that of hand extracted juice. Total of 31 and 29 odor compounds were deteced by GC –Olfactometry. Ethyl butanoate, octanal, linalool, and decanal were the major aroma compounds in mechanically extracted juice. The compounds with strong aroma intensity of hand extracted juice were limonene and linalool. The odor profiles showed that the mechanically extracted juice and hand extracted juice mainly presented citrus odor and flowery odor, respectively. Major group odor of mechanically extracted juice presented higher than that of hand extracted juice except fatty note. The results indicated that the process of extraction could change the orange juice aroma, and the contents of octanal and neral with citrus odor could be increased by mechanical extraction.