高粱黄酮的提取及其测定方法的建立

Extraction and Quantitative Determination of Flavonoids in Sorghum

摘要:

选用乙醇作为提取溶剂，对高粱中总黄酮进行提取，研究乙醇浓度、浸提温度、浸提时间、料液比对高粱黄酮提取率的影响。并通过单因素及正交试验，确定了高粱黄酮的最佳提取工艺：乙醇浓度为65%,80℃下浸提2h，料液比为6∶100。此工艺下提取的高粱黄酮含量为0.54%。

Abstract:

Extraction of flavonoids in sorghum using ethanol was studied. The effects of ethanol concentration, temperature, extraction time, and ratio of material to solvent were studied. The optimum conditions of extraction were obtained as follows: 65% ethanol, solid/liquid ratio of 6∶100, extraction time of 120 min under 80℃ under the optimal conditions, the content of total flavonoids was 0.54%.