HPLC-MS/MS法快速测定鲜鱼肉中孔雀石绿及其代谢物含量

Quick Determination of Contents of Malachite Green and it's Metabolites in Fresh Fish Meat by HPLC-MS/MS

摘要:

建立了鲜鱼肉中孔雀石绿及其代谢物隐色孔雀石绿残留量的高效液相色谱-串联质谱测定方法. 采用电喷雾正离子模式离子化,选择反应扫描模式定量,孔雀石绿的定量监测离子对为m/z:329>313,隐色孔雀石绿的定量监测离子对为m/z:331>239,孔雀石绿的检出限为0.0022ng/mL,定量限为0.0073ng/mL；隐色孔雀石绿的检出限为0.0433ng/mL,定量限为0.14ng/mL,线性范围均为1～10ng/mL,相对标准偏差均小于4%(n=6),方法的回收率均大于90%.

Abstract:

A method was established for the determination of contents of malachite green and it's metabolites in fresh fish meat by HPLC-MS/MS.The electrospray positive-ion mode was applied for ionization and the selective ion monitoring mode (SRM) was used for quantification. The quantitative ion pairs of malachite green and leucomalachite green were 329>313 and 331>239. The detection limits of malachite green and leucomalachite green were 0.0022ng/mL and 0.0433ng/mL,and the limits of quantification of green and leucomalachite green were 0.0073 ng/mL and 0.14ng/mL. The liner ranges of the samples both were 1-10ng/mL and RSD were less than 4% (n=6).