气体冲击处理对草莓活性氧代谢影响的多变量解析

Multivariate Analysis of Reactive Oxygen Species Metabolism of Strawberry Treated with Gas Impingement

摘要:

探讨了气体冲击处理对草莓活性氧代谢的影响。单因素方差分析显示,常温下臭氧显著抑制了草莓果实腐烂指数和呼吸强度的升高,而在低温下显著抑制了腐烂指数、丙二醛的上升,氮气则抑制了常温下果实腐烂指数和丙二醛的上升,加速了过氧化氢含量的下降。主成分分析结果也表明,臭氧处理有利于草莓的保鲜。偏最小二乘回归分析得出,腐烂指数与呼吸强度、花青素、脂氧合酶活性、超氧化物歧化酶活性呈正相关,与过氧化氢酶活性、羟自由基清除能力、超氧阴离子清除能力呈负相关；过氧化氢与过氧化物酶活性、多酚氧化酶活性呈正相关。通径分析显示,呼吸强度、DPPH自由基清除能力、羟自由基清除能力、抗坏血酸过氧化物酶活性为草莓腐烂指数的直接作用因子,总酚、还原力、多酶氧化酶活性均对其起负间接作用；谷胱甘肽、超氧化物歧化酶活性、过氧化物酶活性为过氧化氢的直接作用因子,多酚氧化酶活性、抗坏血酸过氧化物酶活性、DPPH自由基清除能力、超氧阴离子清除能力均为过氧化氢的间接作用因子。

Abstract:

The effects of individual treatments with gas impingement on fruit quality and reactive oxygen species metabolism of strawberries were analyzed by one-way analysis of variance, principal component analysis (PCA), partial least squares regression (PLSR), and path analysis (PA). One-way analysis of variance showed that ozone significantly inhibited the strawberry fruit rot index and respiration intensity at room temperature, while suppressed decay index as well as the methane dicarboxylic aldehyde (MDA)content during storage at 1℃. Nitrogen inhibited the decay index and MDA content of strawberries stored at 20℃.It was supported by PCA results which concluded that the ozone treatment was helpful for strawberries storage. PLSR indicated that decay index was positively correlated with respiration intensity, anthocyanins, LOX, and SOD activity, but negatively correlated with CAT, radical scavenging ability of hydroxyl, and ultra oxygen anion. Hydrogen peroxide was positively correlated with POD and PPO. It was showed by PA that respiratory intensity, DPPH radical scavenging ability, hydroxyl radical scavenging ability, and APX were all direct factors for strawberry decay index, while total phenol, reducing power and PPO had negative indirect effects on decay index. GSH, SOD and POD directly impacted hydrogen peroxide of strawberry fruits, but PPO, APX, DPPH radical scavenging ability, and ultra oxygen anion removal ability were indirect factors of hydrogen peroxide.