体外酶法水解制备小分子透明质酸

Preparation of Small Molecular Hyaluronan by Enzymatic Hydrolysis in Vitro

摘要:

为了制备分子量分布集中的小分子透明质酸,在6g/L的高分子量透明质酸水溶液中加入一定梯度重组水蛭透明质酸酶,并控制水解时间。结果表明,通过控制加入重组水蛭HAase的量和水解时间,根据水解分子量下降规律,可以制备出分子量分布集中的小分子HA,包括水解终产物透明质酸四糖和六糖。同时,通过高效体积排阻色谱和琼脂糖凝胶电泳,可以证明水解产物的分子量分布集中。

Abstract:

In order to get concentrated distribution of small molecular hyaluronan, 6g/L of high molecular hyaluronic acid aqueous solution was prepared, added with a certain gradient of hyaluronidase, and controlling the hydrolysis time. The results showed that controlling the amount of hyaluronidase and hydrolysis time, particular molecular weight hyaluronic acid could be prepared according to the law of the hyaluronan molecular weight decreased,which included tetrasaccharide and hexasaccharide. Furthermore,through high performance size exclusion chromatography and agarose gel electrophoresis, we could prove that the molecular weight distribution of hydrolysate was concentrated.