油茶粕中茶皂素连续多级逆流水提工艺的建立

Establishment of Continuous Multi-Stage Countercurrent Water Extraction Process of Tea Saponin from Camellia Meal

摘要:

为提高油茶粕经济价值,充分利用油茶粕资源,以油茶粕为原料,采用热水连续多级逆流提取油茶粕中的茶皂素,优化了单级提取和连续多级逆流提取工艺参数。结果表明:单级提取的优化条件为提取时间85min,提取温度80℃,料液比1∶16g/mL,油茶粕提取粒度60~100目。在单级提取基础上,确定连续多级逆流提取的优化提取级数为3级,每级优化的提取时间为20min,此时得到的提取液中茶皂素的质量浓度为33.14mg/mL,比单级提取质量浓度提高了1.23倍,提取时间缩短了29.41%,提高了提取效率。 水溶液结合连续多级逆流提取,获得了质量浓度较高的茶皂素,并且缩短了提取时间,提取流程绿色环保,更易实现工业化生产。

Abstract:

In order to improve the economic value of camellia meal and make full use of the camellia meal resources, tea saponin was extracted from camellia meal by continuous multi-stage countercurrent extraction process using hot water, and the parameters of single-stage extraction and continuous multi-stage countercurrent extraction process were optimized. The results showed that the best conditions for single-stage extraction were as follow:extraction time 85 min, extraction temperature 80℃, material-liquid ratio 1∶16g/mL, and the most suitable extraction size of camellia meal 60-100mesh. On the basis of single-stage extraction, the optimal number of extraction stages for continuous multi-stage countercurrent extraction was determined to be 3 stages, and the optimum extraction time for each stage was 20min. Under those conditions,the content of tea saponin in the extract was 33.14mg/mL, which was 123% higher than that of single-stage extraction. Extraction time was shortened 29.41% and extraction efficiency was improved at the same time. In this study, an aqueous solution combined with continuous multi-stage countercurrent extraction was used to obtain tea saponin with a higher mass concentration, and the extraction time was shortened. The extraction process was green and environmentally friendly, and industrial production was easier to achieve.