黑果枸杞花青素提取物对大鼠急性痛风性关节炎的作用

Effect of Anthocyanin Extract of Lycium ruthenicum Murr. on Rats with Acute Gouty Arthritis

摘要:

为研究黑果枸杞花青素提取物(anthocyanin extract of Lycium ruthenicum Murr., AEL)对急性痛风性关节炎(acute gouty arthritis, AGA)大鼠的作用,将60只雄性SD大鼠,随机分为正常对照组、模型对照组、阳性对照组、AEL高剂量组(238.0mg/kg)和AEL低剂量组(59.5mg/kg),每组12只,分为2批,每批6只。第一批动物灌胃2周,第二批动物灌胃4周后,采用50mg/mL微晶型尿酸钠(microcrystalline sodium urate, MSU) 0.2mL注射到大鼠右踝关节诱导大鼠急性痛风性关节炎模型,评价AEL对AGA大鼠踝关节肿胀和跛行步态的改善效果。所有动物继续灌胃至5周,采用质量分数2.5%的氧嗪酸钾饲料喂食诱导高尿酸血症模型,测定造模2h、3h后大鼠的血清尿酸水平。研究发现,与模型对照组比较,灌胃2周,AEL高剂量能明显缓解大鼠造模6h后踝关节肿胀,改善跛行步态；灌胃4周,AEL高、低剂量均能明显缓解大鼠造模后6h、24h踝关节肿胀和改善造模6h后跛行步态,AEL高剂量还能明显改善造模24h后跛行步态；灌胃5周后,AEL高、低剂量均能明显抑制氧嗪酸钾导致的大鼠血清尿酸升高。结果表明,AEL能从抗炎和降尿酸两个方面对大鼠急性痛风性关节炎表现出明显改善作用。

Abstract:

To investigate the prevention effect of anthocyanin extract of Lycium ruthenicum Murr. (AEL) on rats with acute gouty arthritis (AGA), 60 male SD rats were randomly divided into 5 groups, including normal control group, model control group, positive control group, AEL high dose group (238.0mg/kg) and AEL low dose group (59.5mg/kg). There were 12 rats in each group. Animals in each group were divided into 2 batches with 6 rats in each batch. After gavage for 2 weeks in the first batch of animals and 4 weeks in the second batch of animals, the rat model of AGA induced by injecting 0.2mL of 50mg/mL microcrystalline sodium urate into the right ankle of rats, and the effect of AEL on ankle joint swelling and claudication gait of AGA rats were evaluated. After continued gavage for 5 weeks, hyperuricemia model was induced by feeding 2.5% potassium oxazinate. The serum uric acid level of rats was measured after modeling at 2h and 3h. It was found that compared with the model control group, high dose of AEL after gavage for 2 weeks could significantly alleviate the ankle joint swelling and improve the claudication gait of rats at 6h after modeling. After gavage for 4 weeks, both high and low doses of AEL could significantly alleviate the ankle joint swelling at 6h and 24h and improve the claudication gait of rats at 6h after modeling, and high doses of AEL could also significantly improve the claudication gait at 24h after modeling. After 5 weeks of gavage, both high and low doses of AEL could significantly inhibit the increment of serum uric acid induced by potassium oxazinate. The results showed that AEL could significantly improve AGA in rats through anti-inflammatory effect and reducing uric acid.