ZnO-PDMS超双疏薄膜对腐败希瓦氏菌生物被膜的抑制机制

Antibiofilm Mechanism of ZnO-PDMS Superamphiphobic Films Against Shewanella putrefaciens

摘要:

为提高食品贮藏加工材料表面对水产品腐败希瓦氏菌生物被膜的抗黏附性能,以聚二甲基硅氧烷(PDMS)为成膜剂,以水热合成法制备的不同微观形貌的ZnO微纳米粒子为改性剂,采用流延法制备了ZnO-PDMS薄膜,并研究了薄膜对腐败希瓦氏菌生物被膜的抑制性能及作用机理。结果表明:改性ZnO微纳米粒子的微观形貌影响PDMS薄膜的疏水疏油性能,以绒球状和花状ZnO微纳米粒子为改性剂,可获得ZnO-PDMS超双疏薄膜,对水和油的接触角均大于150°。薄膜的抗菌黏附性能与疏水疏油性能呈正相关,随着培养时间的延长,ZnO薄膜释放的Zn²⁺破坏了细菌细胞膜,并抑制胞内ATP酶和AKP酶活性,引起细菌代谢紊乱导致细菌死亡。相较于棒状和绒球状微纳米粒子改性的PDMS薄膜,花状ZnO-PDMS薄膜具有更强的抗黏附性能和抑菌效能。同时具有超双疏性能和抗菌性能的ZnO-PDMS薄膜可有效抑制腐败希瓦氏菌生物被膜的形成和生长,研究旨在为抗生物被膜材料的研制提供技术支持。

Abstract:

To improve the surface anti-adhesion property of food storage and processing materials against Shewanella putrefaciens biofilm of aquatic products, the ZnO-PDMS films were prepared by tape casting method with polydimethylsiloxane (PDMS) as filmogen, ZnO micro-nanoparticles with different micro-nanophology prepared by hydrothermal synthesis method as modifiers, and antibiofilm property and mechanism of the ZnO-PDMS films against Shewanella putrefaciens biofilm were studied. The results showed that the hydrophobic and oleophobic properties of PDMS films were affected by micromorphology of modified ZnO micro-nanoparticles. Using pompon-shaped and flower-shaped ZnO micro-nanoparticles as modifiers, ZnO-PDMS superamphiphobic films were prepared, and their contact angles of water and oil were both beyond 150°. There was a positive correlation between antibacterial adhesion and amphiphobic of the films. With the extension of culture time, Zn²⁺ released from ZnO film, damaged the bacterial cell membrane and inhibited ATP and AKP enzyme activity in the cell, resulting in bacterial metabolic disorder and death. Compared with PDMS films modified by rod-shaped and pompon-shaped micro-nanoparticles, flower-shaped ZnO-PDMS film had stronger anti-adhesion and antibacterial property. Formation and growth of Shewanella putrefaciens biofilm could be effectively inhibited by the ZnO-PDMS film with both surper-amphiphobic and antibacrobic properties. This study aimed to provide technical support for development of antibiofilm materials.