D-阿洛酮糖的高效生物催化合成研究进展

Research Progress on Efficient Biocatalytic Synthesis of D-Allulose

摘要:

D-阿洛酮糖是一种新型的具有降血糖、抗氧化等特殊生理功能的低热量稀有糖。目前,D-阿洛酮糖的生物合成主要是利用D-阿洛酮糖3-差向异构酶来催化D-果糖生成D-阿洛酮糖,利用生物酶法大规模合成D-阿洛酮糖的研究重点主要集中在提升差向异构酶的催化活性、热稳定性和耐酸性以满足工业生产的需求。介绍了D-阿洛酮糖的生产现状及其生物酶法合成的最新进展,包括异构酶的分子定向改造、辅助定向进化的超高通量筛选方法、新型酶分子的固定化策略等以提升差向异构酶的工业属性,并进一步探讨了全细胞催化系统在D-阿洛酮糖生产中的应用。研究旨在为D-阿洛酮糖的高效生物催化合成提供理论参考,并提出工业生产中存在的挑战和潜在的解决方案。

Abstract:

D-allulose is a novel low-calorie rare sugar with special physiological functions, including blood sugar regulation and antioxidant effects. In present, the biological synthesis of D-allulose mainly utilized D-allulose 3-epimerase to catalyze the conversion of D-fructose into D-allulose. The large-scale biosynthesis of D-allulose using biological enzymes has primarily focused on improving the catalytic activity, thermal stability, and acid tolerance of D-allulose 3-epimerase to meet industrial production requirements. The research progress on the current status of D-allulose production and the latest advances in its biosynthesis, including molecular-directed modification of isomerases, ultra-high-throughput screening assisted by directed evolution, and novel enzyme immobilization strategies to enhance the industrial properties of D-allulose 3-epimerase were summarized. Furthermore, the application of whole-cell catalytic systems in D-allulose production was also discussed. The aim of this review was to provide a reference for the efficient and green biosynthesis of D-allulose and to propose challenges and possible solutions in industrial production.