高效液相色谱法测定葡萄酒中人工合成色素

Determination of Synthetic Food Pigments in Port Wine by HPLC

摘要:

建立了葡萄酒中柠檬黄、苋菜红、胭脂红、日落黄含量的高效液相色谱法测定方法. 采用ODS-Cl8色谱柱，甲醇和002mol/L乙酸铵的水溶液为流动相进行梯度洗脱，流速为08mL/min,采用紫外检测器，检测波长为470nm. 该方法测定结果的相对标准偏差小于3%-n=6-,平均回收率大于95%.

Abstract:

A method for the determination of tartrazine, amaranth, ponceau, and sunset yellow in wines by high performance liquid chromatography -HPLC- was established. The separation was achieved on a ODS-C18 column by a gradient elution with methanol and 002mol/L aqueous ammonium acetate as mobile phase. The flow speed of the mobile phase was 08mL／min and the determination was conducted at 470nm through a UV detector. The average recovery of the method of real samples was more than 95%, and the RSD was less than 3%. Therefore, the method is suitable for the experiment.