三氯乙酸沉淀蛋白法辅助提取牛奶中环腺苷酸工艺

Trichloroacetic Acid Protein Precipitation Assisted Extraction of cAMP in MilkL

摘要:

以牛奶为原料，通过离心去除乳中脂肪，采用不同质量分数不同用量TCA沉淀乳中蛋白质，再浓缩乳糖，分离提取环腺苷酸（cAMP） ，选用最佳高效液相色谱条件测定牛奶中cAMP含量. 结果表明，不同质量分数TCA处理组之间比较，质量分数为10%TCA处理组cAMP含量显著高于其他2组（P<0.05）；不同用量TCA处理组之间比较，8mL相同质量分数TCA处理组cAMP含量较其他2组显著降低（P<0.05）. 选用TCA沉淀乳蛋白方法提取cAMP时最佳工艺是TCA质量分数为10%，用量为4mL, cAMP质量浓度为（29.02±1.58）μg/mL. 研究通过建立牛奶中cAMP的提取工艺，可从奶酪副产物、乳清、不合格原料奶中提取cAMP，在降低cAMP提取成本、废物利用、增加牛奶附加值等方面具有重要意义.

Abstract:

With milk as raw material, fat in milk was removed by centrifugation and protein was precipitated using different concentrations and dosages TCA. Afterward, the lactose was concentrated and cAMP was extracted. The best conditions of high-performance liquid chromatography(HPLC） were obtained to determine the content of cAMP in milk. The results showed that the cAMP content in the 10% TCA treatment group was significantly（P<0.05） higher than the other two groups with different TCA concentrations. The cAMP content in the highest-dosage （8mL）TCA treatment group was significantly lower（P<0.05） than that in the other two TCA treatment groups with the same concentration and different dosages. The highest cAMP content（（29.02±1.58）μg/mL） was obtained under the condition of 4mL of 10% TCA. In this study, the extraction method of cAMP from milk was established and could be used to extract cAMP from by-product of cheese, whey, and unqualified raw milk, which had great significance in reducing the extraction cost of cAMP, utilizing waste materials, and increasing the additional value of milk.