胶体金测试条快速定量测定呕吐毒素技术在粮食中的应用

Study on Rapid Quantitative Detection for Vomitoxin in Grain with Colloidal Gold Strips Method

摘要:

采用胶体金测试条法对粮食中呕吐毒素进行快速定量检测，结果表明，呕吐毒素的检测限为118μg/kg，定量限为316μg/kg, 500～5000μg/kg质量浓度范围内，相关系数r为0.9987. 采用有证实物参考物质进行比较，正确度为90.3%～116.6%，变异系数为47%～19.6%. 采用配对t-检验，与高效液相色谱法的测定结果相比较，二者无显著差异.

Abstract:

The colloidal gold strips method was used for the rapid quantitative detection of vomitoxin in grain. The results showed that the detection limit of vomitoxin was 118μg/kg. The quantitation limit was 316μg/kg and the correlation coefficient （r） was 0.9987 in the range of 500-5000μg/kg. Compared with the certified reference material, the accuracy was in the range of 90.3%-116.6% and the coefficient of variation was ranged from 4.7% to 19.6%. There were no significant differences between the colloidal gold strips and the high-performance liquid chromatography using the paired t-test.