黑曲霉M8固态发酵槐米对高尿酸血症小鼠的影响

Effects of Flos Sophorae’s Solid-state Fermention by Aspergillus niger M8 on Hyperuricemia Mice

摘要:

以槐米为原料进行黑曲霉固态发酵，HPLC法测定槐米发酵前后槲皮素质量分数变化，研究了槐米发酵物对高尿酸血症小鼠的血尿酸-SUA- 、尿素氮-BUN- 、血肌酐-SCr-水平的影响. 结果表明：经黑曲霉M8菌株固态发酵后，槐米中有9562%的芦丁转化为槲皮素，槲皮素质量分数由发酵前3 28mg/g提高到3923mg/g，是发酵前的1196倍. 槐米发酵物高剂量组-400mg/kg-和低剂量组-200mg/kg-可分别使高尿酸小鼠SUA值降低2437%, 1796%，与模型组相比，有显著性差异-P <0 05- ；而未发酵的槐米组-400 mg/kg-仅使小鼠SUA值降低223%，与模型组相比，无显著性差异- P >0 05- ，表明黑曲霉发酵槐米的降尿酸作用效果优于未发酵槐米. 此外，槐米发酵物能显著降低高尿酸血症模型小鼠BUN、SCr值-P<0 05- ，可减轻造模药物对小鼠肾功能的损伤. 急性毒理实验证明，槐米黑曲霉发酵物安全.

Abstract:

Flos Sophora was used as a raw material for solid-state fermentation by Aspergillus niger M8, and changes of quercetin contens in Flos Sophora before and after being fermented were tested by HPLC. The results showed that after solid-state fermentation of Flos Sophora by Aspergillus niger M8, most of the rutin -95 62%- was transformed to quercetin and the quercetin content was increased from 3 28 mg/g to 39 23 mg/g, which was 11 96 times higher than unfermented Flos Sophora . The serum uric acid -SUA- content of mice treated by different doses -400 mg/kg and 200 mg/kg- of fermented Flos Sophora was decreased 24 37% and 17 96%, which was significantly - P <0 01- lower than that in the model group. However, the SUA content of mice treated by the high does of unfermented Flos Sophora -400 mg/kg- was only 2 23% lower than that of the control group. The results demonstrated that the solid-state fermentation by Aspergillus niger M8 could improve the reducing-SUA activity of Flos Sophora . Moreover, the blood urea nitrogen -BUN- and serum creatinine -SCr- contents in fermented Flos Sophora treatment groups were significantly - P <0 05- lower than these in the model group, which indicated that fermented Flos Sophora could reduce the injury of drugs on mice kidneys. In addition, the safety of the product was tested by the acute toxicological experiment